ProductDescriptionSize:100μlAbType:PolyclonalReactivity:AllImmunogen:Methylated-KLHHost:Rabbit

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ProductDescriptionTherabbitpolyclonalantibodywasproducedbyimmunizinganimalswithmethylated-KLHconjugates.AntibodywaspurifiedwithproteinA-conjugatedagarosefollowedbymethyllysineagaroseaffinitychromatography.SpecificityThepananti-mono-anddimethyllysineantibodydetectsproteinspost-translationallymodifiedbymono-anddimethylationonlysineresidues,butnotthetrimethyllysine,acetyllysine,orunmodifiedlysineresiduesinawiderangeofsequencecontexts.RecommendedApplicationsELISA,WB,IAP*WB=Westernblotting;IAP=Immunoaffinitypreciptation.RecommendedDilutionWB:1:1000;IAP:1:200.IMPORTANTNOTESForWesternblotting,incubatemembranewithdilutedantibodyin5%BSA,1xTBS,0.1%Tween-20at4℃withgentleshaking,overnight.Optimaldilutions/concentrationsshouldbedeterminedbytheenduserandmaybeassaydependent.

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A.Dotblottinganalysisonindicatedamountoflysinemethylated-KLHconjugates(Lane1),trimethylated-KLHconjugates(Lane2),acetylated-KLHconjugates(Lane3),andunmodifiedKLH(Lane4)usinganti-mono-,dimethyllysinepolyclonalantibody.B.Westernblottinganalysison20µgofcrudeproteinsfromHeLawholecelllysatesusinganti-mono-anddimethyllysinepolyclonalantibody(duplicatedloADIng,1:2000).

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ScientificDescriptionThepost-translationalε-aminolysinemethylationofproteinsisanimportantreversIBLemodificationcontrollingproteinactivity.Thereversiblelysinemethylationofproteinsplaysavitalroleintheregulationofmanycellularprocessesincludingchromatinremodeling,geneexpression,cellproliferation,etc.Mono-anddimethylationaremethylatedformsoflysineandoccurinbothhistonesandnon-histonesubstrates.Methylationoflysineresiduesismodulatedbyspecificcounteractiveenzymesincludinglysinemethylases(KMTs),demethylases(KDMs),histonemethylases(HMTs),andhistonedemethylases(HDMs),whosepathwayshavebecomepromisingtargetsfordiscoveryofanti-cancerdrugs.Storage&StABIlityTheantibodyisdeliveredinlyophilizedpowder.Uponreceipt,pleasecentrifugetheantibodyat12,000xgfor20seconds,andthenreconstituteitwiththeantibodystabilizerprovided.ThestabilizercontainsPBS,50%glycerol,and0.01%sodiumazide.Pleasestoretheantibodyat-20°C.Avoidrepeatedfreeze/thawcycles.Antibodyisstablefor12monthsfromdateofreconstitution.Equilibratetheantibodytoroomtemperaturefor2minutesandmixbygentlypipettingbeforeuse.**Researchpurposesonly.Theproductisnotintendedtobeusedfortherapeuticordiagnosticpurposesinhumansoranimals.

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