ProductDescriptionSize:100μlAbType:MonoclonalReactivity:AllImmunogen:NitratedKLHHost:MOUSE

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ProductDescriptionThemouse-derivedanti-3NTantibodyispurifiedwithProteinG.Itspecificallyrecognizesproteinswithtyrosinenitratedresiduesbutnotthetyrosinephosphorylatedornon-modifiedtyrosineresidues.BesidescommonapplicationsinWesternblottingandimmunoprecipitation,theantibodyhasbeenwellutilizedforproteomicscreeningtotyrosinenitratedsubstratesfromprotease-digestedproteinsbothinprokaryoticandeukaryoticcells.Source/PurificationThisproductisproducedbyimmunizingmicewithnitratedKLH.TheasciteswaspurifiedbyproteinG.SpecificityAllnitratedproteinsexpectedRecommendedApplicationsELISA,WB,IP*WB=Westernblotting; IP=ImmunoprecipitationRecommendedantibodydilutionWB:1:1000ImportantForWesternblotting,incubatemembranewithdilutedantibodyin5%BSA,1xTBS,0.1%Tween-20at4℃withgentleshaking,overnight.Useatanassaydependentconcentration.Optimaldilutions/concentrationsshouldbedeterminedbytheenduser.Notyettestedinotherapplications.Dotblotting analysisonindicatedamountofpeptidesusing3-nitrotyrosinemousemAb.Thelistofpeptidesisincludedinthetableontheright.Westernblot analysison30μgofextractsfromHeLaandMEFwholecelllysates,untreatedortreatedwithperoxynitrite(3mM,30mins)orpervanadate(3mM,30mins),usinganti-3-nitrotyrosinemouseAntibody(FigureB)oranti-phospho-TyrosinerabbitpAb(PTM-702)(FigureC).ScientificDescriptionNitrotyrosineisaproductoftyrosinenitrationmediatedbyreactivenitrogenspeciessuchasperoxynitriteanionandnitrogendioxide.ItisverystableandsoissuitableforanalysisinBIOLOGicalsamplesuchasplasma,lungaspirants-BALF(Bronchoalveolarliningfluid)andurine.IncreasednitrotyrosinelevelshavebeenassociatedwithseveralphysiologicalandpathologicalconditionsandisconsideredaMarkerofNO-dependent,reactivenitrogenspecies-inducednitrativestress.ResearchshowsthatnitrotyrosinelevelscanbereducedbyN-acetylcysteinewhichisaprecursortoglutathione,oneofthebody’sprimaryendogenousantioxidants.Storage&StABIlityTheantibodyisdeliveredinlyophilizedpowder.Uponreceipt,pleasecentrifugetheantibodyat12,000xgfor20seconds,andthenreconstituteitwiththeantibodystabilizerprovided.ThestabilizercontainsPBS,50%glycerol,and0.01%sodiumazide.Pleasestoretheantibodyat-20°C.Avoidrepeatedfreeze/thawcycles.Antibodyisstablefor12monthsfromdateofreconstitution.Equilibratetheantibodytoroomtemperaturefor2minutesandmixbygentlypipettingbeforeuse.**Researchpurposesonly.Theproductisnotintendedtobeusedfortherapeuticordiagnosticpurposesinhumansoranimals.

 ICL免疫学顾问实验室有限公司是由Gerrie A. Leslie博士于36年前在奥斯威戈湖成立的一家咨询公司。ICL的主要功能是定制抗体开发，抗体表征和编写产品概况。ICL目前位于俄勒冈州的波特兰市，现已发展成为一家致力于以批发和零售的方式生产最高质量的一级和二级抗体，免疫测定，抗原和参考标准品的公司。我们的客户包括许多世界领先的IVD制造商，制药公司和研究机构。我们邀请您浏览我们的在线目录，以了解我们如何以最合理的价格帮助您找到理想的试剂。